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A wide variety of assay products for identifying and analyzing of proteins and their functions. Products utilize various detection methods and sample types and can be used for applications including medical, cellular, molecular, and protein research.
-galactosidase (-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of -gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of -gal like O-nitrophenyl--D-galactopyranoside (ONPG). -gal is a cost-effective simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the -galactosidase gene LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
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Recombinant cynomolgus CXCL13 (BCA-1) is a His-tagged chemokine produced in E. coli for use in cell-based and biochemical assays. It acts as a B-lymphocyte chemoattractant and is supplied lyophilized with recommended reconstitution and storage instructions. Purity and bioactivity are validated by SDS-PAGE and cell migration assays.